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EXAMPLES Example 1: CRISPR Complex Activity in the Nucleus of a Eukaryotic Cell Example 2: CRISPR system modifications and alternatives Example 3: Sample target sequence selection algorithm Example 4: Evaluation of multiple chimeric crRNA-tracrRNA hybrids Cell culture and transfection SURVEYOR assay for genome modification Computational identification of unique CRISPR target sites Streptococcus pyogenes SF370): Streptococcus pyogenes SF370): Streptococcus pyogenes SF370): S

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Cell lines were incubated in a 5% CO 2 atmosphere at 37C

In this investigation, we initially identified oridonin as a direct covalent inhibitor of GSH, setting it apart from other drugs that disrupt ROS by targeting proteins both upstream and downstream of GSH